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Live Cell Imaging

Incucyte display

All of the Facility's confocal and wide-field systems have some kind of environmental control; the systems most frequently used for live cell imaging are shown below. Our dedicated live cell imaging platform is a Sartorius Incucyte, which is a wide-field fluorescence microscope specifically designed to fit inside a standard tissue culture incubator.

Our systems:

Sartorius Incucyte SX5

A wide-field fluorescence microscope in an incubator

4x, 10x, 20x objectives

Green/Red and Green/Orange/Near-infrared optical modules

Ideal for longer term live cell imaging experiments running over days or weeks that requiring tightly regulated environmental conditions

Up to 6 separate vessels (multi-well plates, T75s etc), running different acquisition protocols for each vessel.

Common applications include monitoring cell growth and proliferation, expression of reporter genes, spheroid/organoid development, wound healing.

Incucyte
Olympus SpinSR

Olympus SpinSR Spinning Disk Confocal

Pecon full blackout enclosure

Temperature control up to 30°C above ambient

Micro-chamber for gas and humidity regulation

CO2 control from 0.0 Vol-% up to 20.0 Vol-%

O2 control from 0.0 Vol-% up to 21.0 Vol-%

Nikon Ti-2 wide-field

Full enclosure black panel environmental control

Temperature range from 3°C above ambient to 45°C

Temperature measured in the chamber or at the sample

0.1°C Temperature accuracy at sample

Micro chamber for humidity and CO2 

Ti2 oko