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Ultra-high content/ultra-plex imaging

MACSima image

The Imaging Facility's ultra-high content/ultra-plex imaging platform is a Miltenyi MACSima. This system can theoretically image an unlimited number of targets in one sample with the sequential addition, imaging and removal of labelled antibodies. The system is fully automated, uses samples prepared on standard microscope slides and does not require the use of proprietary antibodies.

Our system:

Miltenyi MACSima

Fully automated ultra-high content/ultra-plex imaging platform

Wide-field epifluorescence microscope

Four channel detection (blue, green, red, far-red)

2x, 20x/0.75, 20x/0.45 objectives

Two techniques signal removal: photobleaching of primary conjugated antibodies or antibody release by enzymatic reaction

Automate liquid handling system

Ultraprecise stage for loading sample carriers

Hardware and image-based autofocus

MACS iQ view analysis software for imaging processing (multiplex fluorescence visualisation, segmentation, interactive gating, data analyses, multiple plotting options, workflow editor, distance analyses)

MACSima system